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51.
试验通过对芩术安胎散的急性毒性、亚慢性毒性和临床安全性进行研究,为芩术安胎散对家猫先兆性流产的预防与治疗提供参考。急性毒性试验:制备芩术安胎散药液,取6周龄健康昆明小白鼠60只,随机分为5组,每组12只,第1~4组的给药剂量分别为6 000、4 800、3 840和3 072 mg/kg,对照组给予等量纯净水,10 d内观察有无中毒和死亡,计算半数致死量(LD50);另取6周龄健康昆明小白鼠20只,随机分为2组:试验组给予2.0 g/mL芩术安胎散药液,18 h内灌服3次,每次0.8 mL,对照组给予等量纯净水,给药后饲养7 d,计算最大耐受量(MTD)。亚慢性毒性试验:24只7周龄SD雌性大鼠随机均分为高、中、低剂量组和对照组,在30 d内,每日分别给予4 800、2 400和1 200 mg/kg芩术安胎散,对照组以等量纯净水进行灌胃,每日观察和记录各组大鼠的精神状态、有无中毒症状和死亡;第31天对各组大鼠称重、采血,进行血液学检测,剖检各组大鼠,观察主要脏器有无病变并制作病理切片。临床安全性试验:选取2~5岁健康雌性家猫20只,适应性饲养10 d,随机分组,每组5只,分别为低剂量组(1倍临床推荐剂量:1.15 g/kg)、中剂量组(3倍临床推荐剂量:3.45 g/kg)、高剂量组(5倍临床推荐剂量:5.75 g/kg)及空白对照组,将药物置于胶囊内,口服给药,空白对照组给予空胶囊,每日1次,连续给药7 d,每日观察各组家猫食欲、精神状态及排便情况,于第8天对各组家猫进行静脉采血,检测血常规和血液生化指标。结果显示,急性毒性试验无小鼠死亡,LD50>6 000 mg/kg;小鼠对芩术安胎散的最大耐受量为240 g/kg,表明该受试药物无明显毒性。在亚慢性毒性试验中,各组大鼠的生长发育情况、血常规指标、脏器系数与对照组相比均无显著差异(P>0.05);高、中剂量组与低剂量组、对照组相比血清总胆固醇含量显著下调(P<0.05),除此之外的生化指标均无显著差异(P>0.05)。病理剖检和组织切片观察结果显示,高剂量组大鼠主要组织器官与对照组相比无明显异常。在临床安全性试验中,不同剂量组家猫精神状态、被毛光泽度、粪便情况均正常,血液学指标与对照组相比差异均不显著(P>0.05)。本试验结果表明,中药芩术安胎散无明显毒性,家猫按临床推荐剂量使用是安全的。 相似文献
52.
基于2018年6~9月野外采集的数据,采用野外观测与统计分析相结合的方法,研究了祁连山天祝地区金强河流域典型灌丛:山生柳(Salix oritrepha)、硬叶柳(Salix sclerophylla)、绣线菊(Spiraea salicifolia)、头花杜鹃(Rhododendron capitatum)、金露梅(Potentilla fruticosa)和千里香(Murraya paniculata)灌丛的降雨截留特征,分析了降雨对灌丛的穿透雨量、茎干流量和截留量的影响。结果表明:研究期间共观测到降雨22次,总降雨量为322.2mm。6种灌丛的茎干流量、穿透雨量和截留量与降雨量之间均呈极显著正相关关系(P<0.01),在观测的雨量范围内,茎干流率与穿透雨率随着降雨量的增加而增加,最后趋于稳定,而截留率则随着降雨量的增加而减小,最后也趋于稳定;6种灌丛的茎干流率和穿透雨率随着雨量级的增加而增大,而截留率则随之减小。在所有雨量级中,山生柳的茎干流率最大;在>20mm雨量级中,千里香灌从的穿透雨率最大,绣线菊灌从的截留率最大。 相似文献
53.
紫花苜蓿是世界范围内种植量最广的牧草之一,低温干燥是常用的贮存方法,可有效保存苜蓿当中的营养成分。太阳能干燥箱可以为苜蓿提供干燥环境,保证干燥温度。为此,设计了一种立式箱式结构干燥箱,并对其工作原理、制作方式、内外部的结构形式及保温材料进行了相应的研究。 相似文献
54.
闭式脱粒滚筒的静动态性能分析 总被引:1,自引:0,他引:1
针对联合收割机中闭式脱粒滚筒有可能在外载荷作用下会发生较大变形和在外激励作用下发生共振的问题,采用SolidWorks软件对闭式脱粒滚筒进行了三维建模,以*.x_t格式导入Ansys Workbench建立有限元模型,然后对闭式脱粒滚筒进行静动态性能分析(即静态分析和模态分析)。静态分析结果表明,闭式脱粒滚筒的刚度和强度足够,变形均在设计允许范围之内;模态分析得到的闭式脱粒滚筒前6阶固有频率和振型结果表明,在规定的工况范围内,闭式脱粒滚筒在外激励作用下不会产生共振。研究结果为后续样机的设计和改进提供了理论依据。 相似文献
55.
WANG Lei LV Yang ZHANG Yue XIE Jia-qian ZHAO Di YAN Li-qiong MEI Hui-min WANG Lei YI Dan CHEN Hong-bo DING Bin-ying HOU Yong-qing WU Tao 《中国畜牧兽医》2016,43(5):1355-1360
The probiotics tested in the experiment were isolated from the intestinal of weaning piglets.The isolated probiotics and E.coli K88 were inoculated into the culture of intestinal porcine epithelial cell-1 (IPEC-1).The activity of lactate dehydrogenase (LDH) in the supernatant was measured after incubating for 2.5 h.At the same time, the probiotics and E.coli K88 were co-cultured in vitro, the number of E.coli K88 was counted and the probiotics which could be resistant to the E.coli K88 were selected 2.5 h later.The results showed that the Lactobacillus casei and Bacillus coagulans could significantly reduce LDH activity (P<0.05), decrease the damage of E.coli K88; Bacillus coagulans could inhibit the growth of E.coli K88.At the same time, Bacillus coagulans could resist high temperature, acid and bile salt.The results showed that Bacillus coagulans strains had great potential as the application of probiotics strains.The methods could be used as a model of screen probiotics which could inhibit the growth of E.coli K88 in vitro. 相似文献
56.
Effect of farm characteristics and practices on hygienic quality of ovine raw milk used for artisan cheese production in central Italy 下载免费PDF全文
Elisa Carloni Annalisa Petruzzelli Giulia Amagliani Giorgio Brandi Francesco Caverni Piermario Mangili Franco Tonucci 《Animal Science Journal》2016,87(4):591-599
A survey on ovine dairy farms directly transforming own‐produced milk, in the Italian Marche region, was carried out to assess flock and milking practices that may influence milk hygienic‐sanitary conditions. A census survey established that 24 dairy farms were located in this region. Bulk milk samples were collected throughout the milking period in each dairy farm in 2013. Analyzed variables were: (i) chemical parameters such as fat, protein and lactose content, dry matter and pH; and (ii) total bacterial (TBC) and somatic cell counts (SCC). Chemical parameter values were in agreement with published data while, geometric mean (GM) log10 SCC was 5.91 and TBC GM was 57 978 colony forming units/mL, in compliance with Eropean Union criteria. A positive correlation was found between SCC and TBC when GMs of all farm data were considered (Spearman's rho = 0.7925; P = 0.0001). Statistical analysis did not show significant correlation between SCC or TBC GM and dairy farm principal characteristics. Although SCC levels detected in the present study should suggest the need to implement mastitis control programs, Marche's dairy sheep flocks revealed a good hygienic condition level. This is an important aspect in implementing safety for end users of the final product. 相似文献
57.
58.
Intorre L Meucci V Di Bello D Monni G Soldani G Pretti C 《Journal of the American Veterinary Medical Association》2007,231(4):590-595
OBJECTIVE: To determine tolerance of goldfish and zebrafish to benzalkonium chloride, formalin, malachite green, and potassium permanganate. DESIGN: Tolerance study. ANIMALS: Adult goldfish (Carassius auratus) and zebrafish (Danio rerio). PROCEDURES: Groups of fish (n = 10/group) were exposed to each disinfectant at the therapeutic dosage; at 0.25, 0.5, 3, and 5 times the concentration used for the therapeutic dosage; and at the concentration used for the therapeutic dosage but for 3 or 5 times the recommended exposure time. RESULTS: In both species, exposure to malachite green at the therapeutic dosage resulted in toxic effects, including death. Exposure to formalin at the therapeutic dosage resulted in toxic effects in goldfish, but not zebrafish, and exposure to potassium permanganate resulted in toxic effects in zebrafish, but not goldfish. On the basis of the ratio of therapeutic dosage to median lethal dosage, in goldfish, formalin was more toxic than benzalkonium chloride, which was more toxic than malachite green, which was more toxic than potassium permanganate. In zebrafish, potassium permanganate was more toxic than formalin and benzalkonium chloride, which were approximately equally toxic and more toxic than malachite green. Extending treatment time increased the toxicity of potassium permanganate in zebrafish and the toxicity of formalin and malachite green in goldfish, but did not alter the toxicity of the other disinfectants. CONCLUSIONS AND CLINICAL RELEVANCE: Results indicated that there was no consistency between zebrafish and goldfish in their tolerance to disinfectants, and that therapeutic dosages reported in the literature for these disinfectants were not always safe. 相似文献
59.
Scacchia M Sacchini F Filipponi G Luciani M Lelli R Tjipura-Zaire G Di Provvido A Shiningwane A Ndiipanda F Pini A Caporale V Hübschle OJ 《The Onderstepoort journal of veterinary research》2007,74(3):251-263
Contagious bovine pleuropneumonia (CBPP), caused by Mycoplasma mycoides var. mycoides small colony (MmmSC), is one of the most important diseases of cattle in Africa. The role of innate or acquired cell mediated and humoral immunity in conferring protection against MmmSC infection has not yet been elucidated. On the other hand, the pathological lesions caused by the aetiological agent have been considered indicative of an immunopathological process. In this study ten na?ve cattle were exposed to in-contact infection with animals infected by intubation with a strain of MmmSC. Clinical signs, antibody response, IFNgamma release and pathological changes at necropsy were analysed and compared with the events following in-contact infection of an equal number of animals kept under daily treatment with cyclosporine for the entire observation period of 84 days. Cyclosporine is a suppressor of the immune response related to the T-cell system. Under the conditions of the experiment, cyclosporine appeared to condition the pathogenesis of CBPP by delaying the events that follow infection, bringing further support to the possibility that the immune response may have an impact on the disease outcome. 相似文献
60.
Farnaz Kordbacheh Charles L. Mohler Alan G. Taylor Anna S. Westbrook Hamid Rahimian-Mashhadi Hassan M. Alizadeh Antonio DiTommaso 《Weed Research》2023,63(1):34-44
Abutilon theophrasti (velvetleaf) is a widespread and problematic annual weed. Greenhouse and field experiments were conducted to investigate the effects of different cutting methods on the viability of A. theophrasti seeds. Three cutting methods were assessed: (1) Entire plant cut and dried (EPD)—plants were cut at soil level and dried with capsules attached on the greenhouse bench or soil surface for 4 weeks; (2) capsules detached and dried (CD)—capsules were removed from plants and dried for 4 weeks; and (3) capsules detached and tested while fresh (CF)—a control treatment. Before drying, the developmental stage (stage one, dark green; stage two, light green; stage three, yellowish-green; or stage four, black with the slightly open capsule) and age (days after flowering, DAF) of each capsule was recorded. Seed viability was measured immediately in the CF treatment and after the 4-week drying period in the EPD and CD treatments. No seeds in the EPD and CD treatments were viable when harvested at the first developmental stage (1–8 DAF) in either experiment, but 100% of seeds in the CF treatment in the field were viable when harvested at 8 DAF. In both greenhouse and field experiments, seeds attained full viability at earlier harvest ages in CF than in EPD or CD treatments, suggesting that seeds might become viable relatively early in development but lose viability if allowed to dry. These findings could be applied to optimise late-season mechanical control of A. theophrasti. 相似文献